高效液相色谱法测定血清中富马酸奎的平浓度

Determination of quetiapine fumarate level in serum by HPLC

目的 :建立用高效液相色谱法 (HPLC)测定血清中富马酸奎的平浓度的方法。方法 :HPLC测定 ,流动相为甲醇 水 1mol·L- 1醋酸铵 5mol·L- 1氨水 ( 4 0 0∶10 0∶5∶1)的混合液体 ,pH9.7,流速 1mL·min- 1,C18柱 ,紫外检测波长 2 54nm ,以外标法定量。结果 :线性范围 10～ 3 0 0 μg·L- 1,最低检测限 5μg·L- 1,平均提取回收率 93 .10 % ,日内与日间精密度平均分别为3 .41%和 4.16 %。结论 :用HPLC可以较好的对血清中富马酸奎的平进行定性和定量。

OBJECTIVE To establish an HPLC assay method for the determination of quetiapine fumarate level in human serum. METHODS Using a mixture liquid of methanol:water:1 mol·L -1 ammonium acetate:5 mol·L -1 NH 4OH(400∶100∶5∶1) as mobile phase, pH 9.7 , flow rate was 1 mL·min -1 , C 18 column, the wave length of detection was 254 nm, quetiapine fumarate level was determined by means of external standard method.RESULTS A linearity was obtained from 10～300 μg·L -1 of Quetiapine Fumarate level in serum with a good correlation( r = 0.9997 ). Detection limit was 5 μg·L -1 . The average extraction recovery was 93.10% . The cofficients of inter day and intra day variations were 3.41% and 4.16% respectively. CONCLUSIONS Using HPLC can be used for the detection of quetiapine fumarate in human serum.