肺癌组织中结核杆菌的间接原位巢式PCR法检测

Detection of Mycobacterium tuberculosis in lung cancer tissue by indirect in situ nested PCR

目的明确结核杆菌在肺癌组织中的细胞定位。方法采用敏感特异的间接原位巢式PCR对15个经液相巢式PCR检测示TB-DNA阳性的肺癌组织蜡块进行再次检测。结果结核杆菌典型的杆菌型主要位于癌组织周围间质或部分吞噬细胞胞质内;变异体L型则呈现颗粒状,主要位于癌组织周围增生的肺泡上皮细胞胞质、炎症细胞胞质和吞噬细胞胞质,且呈片状或灶状分布。结论结核杆菌感染可能在肺癌的发病过程中起重要作用。

O bj ective To localize Mycobacterium tuberculosis DNA (TB-DNA) in lung cancer tissue and to investigate the possible relationship of this bacterial infection with the development of lung cancer. Methods A sensitive and specific indirect in si tu nested PCR (ISNPCR) was used to identify and localize TB-DNA in 15 formalin f ixed paraffin-embedded lung cancer tissue specimens, which had been demonstrate d to be positive for TB-DNA by conventional PCR. Results Positive granules of T B-DNA in brown color was found mainly in the cytoplasm of the alveolar epithelia l cells, pulmonary macrophages, in-flammatory cells and a few tumor cells within lung cancer tissues. Conclusion Mycobacterium tuberculosis infection may play a role in the pathogenesis of lung cancer.