电击死心肌组织中纤维连接蛋白的实验观察

Experimental studies on fibronectin expression in myocardium taken from rats died of electrocution

目的 研究电击死大鼠心肌组织中纤维连接蛋白的表达,为电击死和电损伤的鉴定提供一定依据。方法 取大鼠12只,实验组与对照组各6只。应用SABC法对实验组与对照组进行免疫组化研究。同时应用常规染色作比较。结果 电击死心肌内出现纤维连接蛋白的阳性,与对照组差别有意义(P<0.01)。结论 心肌组织中纤维连接蛋白的强阳性表达对电击死的法医学鉴定有一定参考价值。

Objective To establish an immunohistochemical method for postmortem diagnosis of electrocution. Method 12 rats were divided into two groups,experimental and control. In the experimental group,6 rats were killed by electricity,and in the control group,6 rats were killed by breaking the rats' neck. Myocardium sections were then made and were stained by SABC immunohistochemical method as well as HE.Results The expression of the fibronectins in the experimental rats' myocardia were significantly increased as compared with those in the control rats (P <0.01). Conclusion The FN immunohistochemical staining method might be a valuable method used for postmortem diagnosis of electrocution.