大鼠急性心肌缺血zif/268蛋白表达及其意义

Expression and significance of zif/268 protein in acute myocardial ischemia area in rats

目的 探讨急性心肌缺血早期不同时间不同区域心肌细胞内原癌基因蛋白zif/268的表达变化,为心肌早期缺血死后诊断提供新指标。方法 建立心肌早期缺血模型,大鼠分为正常、缺血组。采用免疫组化SABC法研究心肌细胞核蛋白zif/268的累积情况。结果 缺血 60min后,缺血区大鼠心肌细胞有部分心肌细胞核呈弱阳性着色,以后随缺血时间延长核阳性增强。正常和缺血30min组及未缺血区心肌细胞核未见有阳性反应。HE染色无明显病理改变。结论 免疫组化染色法检测心肌细胞核zif/268的表达对急性心肌缺血的死后诊断是一种有价值有意义的手段。

Objective To investigate the expression of zif/268 protein in area of rnyocardial ischemia and to provide a new method for postmortem diagnosis of ischemia. Method The model of the myocardial ischemia has been established, and the rats were divided into control and ischemia groups. Immunohistochemical SABC method was used to observe alteration of zif/268 protein in nuclei of cardiac myocytes. Results After 60min myocardial ischemia, part of the cardial myocyte nuclei were weak staining and the changes became more severe along with the elongation of the myocardial ischemia. The myocardium in normal control and 30min ischemia groups showed negative staining. No change was observed with HE staining in all groups. Conclusion The data shows that SABC zif/268 may be one important method for diagnosis of myocardial ischemia.