河南食管癌高发区青年与中老年食管癌组织中P53蛋白和Rb蛋白表达

Expression of P53 and Rb protein in esophageal squamous cell carcinomas from the young and old patients at high-incidence area for esophageal cancer in Henan

目的 :探讨食管癌高发区河南省林州市青年 (≤ 4 0岁 )与中老年 (≥ 5 0岁 )食管癌患者的分子学变化特征及其意义。方法 :青年食管癌患者 4 3例 ,中老年食管癌患者 73例 ,采用免疫组化卵白素 生物素 过氧化物酶复合物 (ABC)法和组织病理学方法 ,分析食管鳞癌组织中P5 3和Rb蛋白的表达状况与病变程度和年龄差异之间的关系。结果 :青年食管癌患者食管癌组织P5 3阳性率 (10 /43,2 3% )明显低于同一地区中老年患者 (6 2 /73,85 % ) (P<0 .0 0 1)。青年患者Rb变化阳性率 (15 /43,35 % )亦低于中老年患者 (38/73,5 2 % ) ,但差异无统计学意义 (P >0 .0 5 )。结论

Aim: To characterize the molecular changes and their significance in esophageal squamous cell carcinomas (ESCC) from the young (≤40 years old) and old patients (≥50 years old) at high incidence area for esophageal cancer in Linzhou, Henan. Methods: The subjects were 43 cases of ESCC (≤40 years old) and 73 cases of ESCC (≥50 years old). Immunohistochemical method (ABC) and histopathological method were applied to correlate the expression of P53 and Rb protein in ESCC with lesions and age distribution. Results: Positive immunostaining rate for P53 was much lower in esophageal cancer tissue from the young patients (10/43, 23%) than from older cancer patients (62/73, 85%) ( P <0.001). Although the positive immunostaining rate for Rb in the young cancer patients(15/43, 35%) was lower than that in older patients(38/73, 52%), the difference was not significant ( P >0.05). Conclusion: The present study indicates that there may be different molecular mechanisms for ESCC involved in the young and the older patients in Henan, the high incidence area for esophageal cancer.