周围神经损伤晚期修复后脊髓后角P物质和降钙素基因相关肽变化的定量研究

Quantitative analysis of SP and CGRP in the dorsal horn of rabbit spinal cord following delayed repair of peripheral nerve injury

目的探讨周围神经损伤晚期修复后脊髓后角内P物质(SP)、降钙素基因相关肽(CGRP)变化,以反映脊髓后角的功能状态。方法新西兰兔16只腓总神经切断1年后修复,术后1,3,5月取腰骶段脊髓,切片作SP、CGRP免疫组化染色。采用计算机图像分析对后角内SP、CGRP含量进行定量分析研究。结果失神经1年后,脊髓后角内SP、CGRP含量分别为正常的70%和86%。腓总神经晚期修复后,后角内SP、CGRP先丧失,随后逐步恢复,至术后5月,后角内SP、CGRP含量接近正常。结论周围神经损伤晚期修复后,脊髓后角有再生表现,从神经肽角度证明晚期神经损伤有修复价值。

Objective To assess levels of SP and CGRP in the d orsal horn of rabbit spinal cord afte r delayed repair of peripheral nerve injury to show functional state of the dorsa l horn of spinal cord.Methods Peroneal nerves of 16rabbits after s ection for one year were repaired.Sp inal segments L 7 ～S 1 were removed 1,3and 5months postoperatively.SP and CGRP were labeled using immunohistochemical methods.M easurements to quantitate relative amount of SP a nd CGRP in the dorsal horn gray matter were carried out with the aid of compu ter image analysis sys-tem.Results Contents of SP and CGRP in the dorsal horn were 70%and 86%respectively,co mpared to the normal levels followin g denervation for one year.SP and CGRP levels reduced first,then restored gradually after delayed nerve repair.At 5months postoperative,recovery of SP and CGRP was almost complete in the dorsa l horn.Conclusion The dorsal horn of spinal cord shows e vidence of regeneration following d elayed repair of peripheral nerve injury.It is worthwhile to repair delayed nerve inju ry from the neuropeptides point of view.