急性白血病细胞粘附分子的表达

Expression of cell adhesion molecules in acute leukemia cell

目的 探讨细胞粘附分子基因在急性白血病的发生和髓外浸润中的作用。方法 运用基因芯片和逆转录聚合酶链反应 (RT PCR)技术检测了 2 5例急性白血病神经细胞粘附分子 (NCAM)基因、细胞间粘附分子 1(ICAM 1)基因、血管细胞粘附分子 1(VCAM 1)基因的表达。结果 NCAM、ICAM 1、VCAM 1基因在急性白血病表达显著增高 ,并且有白血病细胞浸润患者粘附分子的基因表达高于无浸润患者 ,基因芯片检测结果与RT PCR结果相同。结论 基因芯片能够为急性白血病的相关基因分析提供特异和可靠的数据。急性白血病的发病和浸润机制可能与骨髓细胞中粘附分子表达升高有关。

Objective To investigate the role of cell adhesion molecule in the development and extramedullary infiltration (EI) of acute leukemia. Methods The expressions of neural cell adhesion molecule (NCAM) gene,intercellular adhesion molecule 1 (ICAM 1) and vascular cell adhesion molecule (VCAM 1) genes in 25 acute leukemia patients bone marrow cells were detected by microarray and reverse transcriptase polymerase chain reaction (RT PCR). Results The expressions of NCAM,ICAM 1 and VCAM 1 gene were significantly higher in acute leukemia cells and leukemia cells with EI than in normal tissues and leukemia cells without EI, respectively,both by cDNA microarray and by RT PCR. Conclusion The cDNA microarray is a powerful technique in analysis of acute leukemia cells associated genes. High expressions of cell adhesion molecule genes might be correlated with leukemia pathogenesis and infiltration of acute leukemia cell.