河南食管癌高发区无症状居民幽门螺杆菌感染分析

Analysis on helicobacter pylori (Hp) infection in pylori from symptom-free subjects at high-incidence area for esophageal cancer in Henan

目的 :比较乙醇中性红法、ELISA法和改良Giemsa法对幽门螺杆菌 (Helicobacterpylori,Hp)检测的价值。方法 :食管癌高发区无症状人群 14 8例 ,取胃窦活检粘膜行乙醇中性红染色、改良Giemsa染色检测Hp ,另留取血清行ELISA法检测Hp IgG。结果 :乙醇中性红法、ELISA法和改良Giemsa法的Hp检出率分别为 4 8.6 %、6 3.5 % ,6 2 .8% ,以ELISA法和改良Giemsa法同时阳性为Hp诊断标准 ,乙醇中性红法的敏感度及特异度分别为 73.0 3%和 88.14 % ,ELISA法分别为 10 0 %和 91.5 3% ,改良Giemsa法分别为 10 0 %和 93.2 2 %。乙醇中性红法检出Hp结果与标准结果间的差异有统计学意义 (χ2 =8.2 5 8,P <0 .0 1) ,ELISA法与改良Giemsa法与标准结果间检出结果显著无统计学意义 (P >0 .0 5 )。结论

Aim: To evaluate the values of ethylalcohol neutral red staining,Giemsa staining and ELISA tests in diagnosis of Helicobacter pylori (Hp) infection.Methods: Hp infection in 148 gastric antral biopsy specimens were detected by ethylalcohol neutral red staining and Giemsa staining. Hp IgG in 148 serum samples were detected by ELISA method.Results: Hp positive detection rates by ethylalcohol neutral red staining, ELISA and Giemsa staining were 48.6%, 63.5%,and 62.8%,respectively. Referring to the standard Hp result ( both Giemsa staining and ELISA positive), the sensitivity and specificity of ethylalcohol neutral red staining were 73.03% and 88.14%, ELISA tests, 100% and 91.53%, and Giemsa staining, 100% and 93.22%, respectively. There was significant difference between the positive cases of ethylalcohol neutral red staining and standard Hp result ( P <0.01),but the positive cases of Giemsa staining and ELISA tests had no significant difference compared with standard Hp result ( P >0.05). Conclusion: Giemsa staining and ELISA tests are ideal methods for Hp infection detection.