碱性成纤维细胞生长因子在乙型肝炎肝组织中的表达

Expression of basic fibroblast growth factor in hepatic tissues of hepatitis B

目的 :研究肝组织中碱性成纤维细胞生长因子 (bFGF)及bFGFmRNA的表达及其与慢性乙型肝炎 (CHB)肝血管增生、改建及肝血管纤维化的关系。方法 :对 1 2 0例CHB及 50只CCl4 诱导肝损伤大鼠肝标本进行bFGF免疫组织化学染色及bFGFmRNA原位杂交 ,并检测了CHB肝组织中α SMA、FN、LN、CoⅣ表达与bFGF的关系。结果 :随着CHB肝细胞变性坏死及肝血管病变加重 ,bFGF在肝血管及肝窦壁表达强阳性率逐渐上升 (P <0 0 1 ) ,部分固缩性肝细胞也示强阳性显色。原位杂交显示 ,bFGFmRNA主要分布于血管纤维化区域肝窦壁及部分肝细胞。同时bFGF在肝组织的表达与α SMA、FN、LN及CoⅣ呈同步关系。结论 :bFGF通过激活血管及肝窦内皮细胞 ,引发肝血管增生及内皮细胞凋亡 ,在细胞外基质等参与下 ,最终导致肝血管纤维化。

Purpose To study the relationship between the expression of basic fibroblast growth factor (bGFG) and bFGF mRNA and hepatic vascular proliferation,reconstruction and fibrosis in chronic hepatitis B (CHB). Methods Immunohistochemical staining for bFGF and hybridization in situ for bFGF mRNA were performed on hepatic tissues of 120 cases of CHB and 50 cases of rats induced by CCl 4. The relationship between the expression of α SMA,FN,LN,CoⅣ and bFGF was also determined. Results The strongly positive rate of the expression of bFGF on hepatic vessels and hepatic sinusoidal walls increased accompanied with the exacerbation of degeneration,necrosis of hepatocytes and hepatic vascular diseases( P <0 01),and partial shrunken hepatocytes also showed strongly positive. The result of hybridization in situ showed that bFGF mRNA mainly distributed on area with vascular fibrosis,hepatic sinusoidal walls and some hepatocytes. The expressions of bFGF and α SMA,FN,CoⅣ were synchronous. Conclusion bFGF can initiate the hepatic vascular proliferation and the apoptosis of endothelial cells through activating vessels and hepatic sinusoidal endothelial cells. And finally induces the formation of hepatic vascular fibrosis,hepatic sinusoidal capillarization and fibrous septum of pseudolobuli with the participation of extracellular matrix.