羧甲基牛膝多糖的制备、结构及生物活性研究

Studies on the Preparation, Structure and Bioactivity of CM-AbPS

在NaOH水溶液中 ,以ClCH2 COOH为羧甲基化试剂 ,对牛膝多糖 (AbPS)进行了羧甲基化 ,粗产物经DEAE Cellulose及SephadexG 2 5两次柱层析纯化 ,得到羧甲基牛膝多糖 (CM AbPS)纯品 ,经高效液相法 (HPLC)及毛细管电泳法 (CE)检测表明具有较好的均一性 .并通过样品甲基化和GC MS分析 ,对羧甲基牛膝多糖糖链中羧甲基的分布进行了研究 ,结果表明羧甲基牛膝多糖中羧甲基主要取代在糖链中呋喃果糖的 4 位上 .该产物具有抗肿瘤活性 .

Achyranthes bidentata Polysaccharides (AbPS) was carboxymethylated by ClCH2COOH in aqueous NaOH and the crude product was purified by successive DEAE-Cellulose and Sephadex G-25 column chromatography to yield homogeneous carboxymethylated product ( CM-AbPS), which was detected by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). By the methylation method and GC-MS analysis, we studied the distribution of carboxymethyl groups in the sugar backbone of CM-AbPS and conclude that the carboxymethyl groups mainly substitute on 4-position of the fructofuranose of the sugar backbone. The product has anti-tumor activity.