牛肾上腺皮质细胞合成蛋白多糖的研究

STUDY OF BOVINE ADRENOCORTICAL CELLS SYNTHETIZE HETEROGENEOUS HEPARAN SULFATE PROTEOGLYCANS

我们利用基础培养下的牛肾上腺皮质束、网状带细胞,通过代谢性标记、特异性酶或化学降解、离子交换色谱、凝胶滤过、免疫沉淀分析等方法,研究了该细胞合成蛋白多糖的性质和分子大小。结果表明该细胞在基础培养条件下合成的^(35)S-标记分子是硫酸乙酰肝素蛋白多糖。这种蛋白多糖表现出明显的不均一性。因为三种分子硫酸乙酰肝素蛋白多糖的分子量分别是230、8.3和330kDa。用链霉蛋白酶水解去角蛋白后,它们各自释放的相应的氨基葡聚糖链为30,2.2,150kDa。

Using35S-sulfate labeling , SDS-polyacrylamide gel electrophoresis, DEAE-Trisacryl ion exchange chromatography, Sepharose C1-6B, Ultrogel ACA 44 gel filtration, immunopreci pitation and specially enzymatical and chemical degradations, we examined the nature and molecular sizes of pro-teoglycans synthetized by bovine adrenocortical zona asciculata-reticularis cells under primary culture. The results showed that these35S^sulfate labeled macromolecules are heparan sulfate proteoglycans. These heparan sulfate proteoglycans expressed cl-early the heterogeneity, because their molecular weights are 230, 8.3 and 330 kDa respectively. After digestion of core protein by pronase, they released correspondingly glycosaminoglycan chains of 30, 2.2 and 150 kDa.