摘要
A solid phase radioimmunoassay for free triiodothyronine in serum was developed based on double-antibody coated tubes. The method was turned out to be reliable with good reproducibility, higher sensitivity and easy performance. The measurable range of FT3 in serum was 1.2 to 38 pmol/L. The mean coefficients of variation within and between assays were 1.79%-3.18% and 4.72%-9.31%, respectively. The FT3 concentrations in euthyroid serum as determined by this method were 2.8 to 7.8pmol/L. The FT3 values determined by this new method correlated well with those measured by a commercial radioimmunoassay （r=0.853）.
A solid phase radioimmunoassay for free triiodothyronine in serum was developed based on double-antibody coated tubes. The method was turned out to be reliable with good reproducibility, higher sensitivity and easy performance. The measurable range of FT_3 in serum was 1.2 to 38 pmol/L. The mean coefficients of variation within and between assays were 1.79%-3.18% and 4.72%-9.31%, respectively. The FT_3 concentrations in euthyroid serum as determined by this method were 2.8 to 7.8pmol/L. The FT_3 values determined by this new method correlated well with those measured by a commercial radioimmunoassay (r=0.853).
关键词
血清
固相
三碘甲状腺原氨酸
放射免疫测定
Free triiodothyronine (FT_3), Solid-phase, Radioimmunoassay, T_3 analog, Doubleantibody
