有氧运动升高脂质清除障碍的ApoE^(-/-)小鼠血脂

Aerobic Exercise Increases Lipid Levels Resulted from Blocked Lipid Elimination in ApoE(-/-) Mice

目的：探讨ＡｐｏＥ在有氧运动调节脂质代谢机制中的作用。方法：应用基因调皮除的ＡｐｏＥ－／－鼠建立运动模型。结 果：对比游泳 １２周（２ｈ ｘ ６ｄ）前后结果。ＡｐｏＥ－／－鼠 ＴＣ（１８． ５±２． ２７ ｍｍｏｌ／Ｌ ｖｓ ２７． ９±４．６３ｍｍｏｌ／Ｌ，Ｐ＜０． ０１）和 ＴＧ （１．０９±０．２６ｍｍｏｌ／Ｌ ｖｓ １． ５２±０．３７ ｍｍｏｌ／Ｌ，Ｐ＜０．０１）显著升高，睾丸总胆固醇水平下降。与 Ｃ５７ＢＬ／６Ｊ对照鼠游 泳前后的变化趋势相反（ＴＣ：１．７６±０．３１ ｍｍｏｌ／Ｌ ｖｓ１．３２±０．３２ ｍｍｏｌ／Ｌ，Ｐ＜０．０５；ＴＧ：０．８６±０．１４ ｍｍｏｌ／Ｌ ｖｓ ０．４５±０．０４３ ｍｍｏｌ／Ｌ，Ｐ＜０．０５）。结论：ＡｐｏＥ－／－鼠运动后血脂升高。反映了运动促进组织脂质动员入血和 ＡｐｏＥ缺 失引起脂质清除障碍的机制；也说明运动调节血脂的关键机制包括ＡｐｏＥ参与的受体依赖脂质清除途径；同时提示 脂质清除障碍时，依靠有氧训练改善血脂状况的效果可能不佳。

To approach relevant mechanism to ApoE in alternation of lipid metabolism induced by aerobic exercise, ApoE deficient mice were used to establish an exercise model programmed by 2h x 6d swimming for 12 weeks. Results show that in contrary to C57BL/6J mice, which developed a more health lipid profile in 12w aerobic exercise, ApoE-/- mice had higher plasma TC and TG levels, a very low HDL proportion and a reduced testis cholesterol content after swimming. The plasma concentrations of TC (18. 5± 2. 27mmol/L vs 27. 9± 4. 63mmol/L, p<0. 01) and TG (1. 09± 0. 26mmol/L vs 1. 52±0. 37mmol/L, p<0. 01) increased significantly in ApoE-/- mice, whereas TC (1. 76±0. 31mmol/L vs 1. 32± 0. 32mmol/L, p< 0. 05) and TG (0. 86 ± 0. 14mmol/L vs 0. 45 ± 0. 043 mmol/L, p < 0. 05) decreased in controlled C57BL/6J mice. Electrophorus is-separated and density-quantified, HDL percentages were 6 1. 4 ± 6. 6 % vs 7 0. 2 ± 7. 5 % (p<0. 05) in controlled c57BL/6J mice, and fluctuated at levels of around 5% in ApoE-/- mice; and the percentages of VLDL+LDL were 38. 2±6. 8% vs 29. 8±7. 5% (p<0. 05) in C57BL/6J mice and remained similar levels (94. 7±3. 2% vs 95. 8± 1. 9% ) in ApoE-/- mice. It is concluded that after 12w swimming, the higher levels of TC and TG in ApoE-/- reflect a promotion of which more lipid is mobilized by aerobic exercise to enter circulation from tissues, and an inhibition of lipid clearance from blood caused by ApoE deficiency. The results suggest that the changes of lipid levels modulated by aerobic exercise may mainly take specific receptor pathway involving ApoE to transfer lipid to tissues, and indicate that aerobic exercise may be an inefficient way to improve lipid profile under condition of blocked lipid clearance from circulation.