6B型肺炎球菌荚膜多糖-破伤风类毒素结合疫苗的制备及其免疫原性

Preparation and Immunogenicity of Pneomococcus Type 6B Capsular Po-lysaccharide - Tetanus Toxoid Conjugate Vaccine

目的 制备6B型肺炎球菌荚膜多糖(6B-PNCPS)-破伤风类毒素蛋白(TT)结合疫苗,并研究其免疫原性。方法6B-PNCPS用溴化氰活化后与己二酰肼形成多糖-酰肼基衍生物,然后在蛋白活化剂碳二亚胺作用下将6B-PNCPS与TT进行共价结合。分别将其结合物及多糖免疫NIH小鼠,用ELISA检测小鼠血清中抗6B-PNCPS抗体。结果6B-PNCPS-TT结合物经凝胶色谱分析显示具有较6B-PNCPS更大的相对分子质量,多糖/蛋白比为1.42-1.66。结合物具有6B-PNCPS的血清学特异性,所诱生的抗6B-PNCPS特异性IgG抗体滴度与6B-PNCPS差异有显著意义。结论 用该法制备6B-PNCPS-TT结合疫苗,具有良好的免疫原性。

Objective To prepare and study the immunogenicity of pneomococcus type 6B capsular polysaccharide (6B - PNCPS) - tetanus toxoid (TT) conjugate vaccine. Methods 6B - PNCPS - IT was activated by Cyangen Bromide (CNBr) and reacted with adipic acid dihydrazide (ADH), then 6B - PNCPS - TT conjugate was prepared by carbodiimide - mediated coupling of 6B-PNCPS with tetanus toxoid (TT) . NIH mice were immunized with 6B - PNCPS - TT conjugate, using pure 6B - PNCPS as control, and the PNCPS antibody titers in the sera of them were detected by ELISA. Results Gel chromatography proved that, compared with that of 6B - PNCPS, the relative molecular weight of 6B - PNCPS - TT was large. The ratio of polysaccharide to protein was 1.42- 1.66. The conjugate vaccine showed the serological characteristic of 6B -PNCPS, and the liters of specific IgG induced by it was significantly higher than that induced by 6B - PNCPS. Conclusion The prepared 6B - PNCPS - TT conjugate vaccine showed good immunogenicity.