摘要
P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay_green ability of transgenic plants was significantly improved; (2) both the seed_setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non_transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.
利用农杆菌介导的遗传转化方法将PSAG12 _IPT导入籼稻品种明恢 6 3。在获得的 6 1个独立的转基因植株中 ,有一些表现出叶片衰老显著延缓 ,对选择的两个纯合转基因株系的小区试验的结果显示 :(1)转基因植株倒三叶的持绿性极显著延长 ;(2 )两个纯合转基因株系比原品种的结实率极显著提高 ,株高极显著降低 ;(3)两个纯合转基因株系的有效穗数比原品种极显著或显著提高。
基金
农业部 948项目 (9610 3 2 )
中华科教基金 (97_0 1_0 4)~~
