摘要
通过对水稻 (OryzasativaL .) 4号染色体一段 32 3kb的序列测定和分析 ,在其中 10 8kb的区域内发现了一个由 14个编码S位点相关的受体样蛋白激酶 (SRK)基因组成的基因簇。RT_PCR实验证明了这 14个基因中有 9个基因表达 ,并且这 9个基因有不同的表达模式 :其中 2个基因主要在生殖器官中表达 ,而另外 7个基因在水稻的营养和生殖器官中均有表达。对这些基因的预测的氨基酸序列进行分析表明他们的细胞外受体部分均和甘蓝的SLG蛋白高度同源 ,而细胞内的激酶区都包含有丝氨酸 /苏氨酸激酶中特异的氨基酸。
We have identified 14 S _locus glycoprotein (SLG)_related protein kinase genes in a 323 kb contig of rice (Oryza sativa L.) chromosome 4 and we detected the transcription pattern of this gene cluster by reverse transcription_polymerase reaction (RT_PCR). RT_PCR results revealed that nine putative genes were transcribed in rice and these genes had the different expression patterns: two genes are expressed predominantly in reproductive tissues while the other seven genes are expressed in both reproductive and vegetative tissues. Analysis of the predicted amino acid sequences demonstrated that the extracellular receptor domains are highly homologous to SLG of Brassica, whereas the cytoplasmic kinase domains contain conserved amino acids present in serine/threonine kinases.
基金
科技部
中国科学院
上海市科委资助项目~~
