治疗用卡介苗抗肿瘤动物实验方法的建立

Establishment of An Method for Studying Antitumor Effect of Therapeutic BCG in Mice

目的 建立治疗用卡介苗在小鼠体内抗肿瘤的药效学实验方法。方法 观察治疗用卡介苗(BCG)对小鼠实体瘤S180和腹水瘤(EC)的抑瘤作用。结果BCG可明显减轻S180荷瘤鼠的瘤重,其瘤重抑制率>30％;可明显延长EC腹水瘤小鼠的生存时间,其生命延长率>75％。其中以12.5％和6.25mg/kg两剂量组的效果较佳。结论 该方法可用于卡介苗抗肿瘤动物药学实验。

Objective To establish a method for studying the antitumor effect of therapeutic BCG in mice. Methods Inject s. c. the therapeutic BCG at different dosages into the mice with solid tumor sarcoma 180(S180) or Ehrlich carcinoma(EC) and observe the inhibitory effect on tumors. Results BCG can signifi-cantly reduce the tumor weight of the mice with S180 .The inhibitory rate to tumor weight was above 30% . BCG can also prolong the survival time of mice with EC. The life prolonging rate was above 75 % . The therapeutic BCG at dosages of 12.5 and 6.25ng/kg showed good curative effect. Conclusion The method can be used for the antitumor pharmaceutical test of BCG in animals. .