马尾松林生态系统中球孢白僵菌种群动态研究

Population dynamics of Beauveria bassiana in masson pine plantation ecosystem.

应用选择性培养基标准平板技术 ,对接种式施放球孢白僵菌的试验区球孢白僵菌种群数量动态进行了调查研究 .监测 1年的结果表明 ,各放菌区在放菌初期的带菌量与初始放菌量密切相关 ,1个月后关系不明显 .放菌初期各宿存部位带菌量的大小依次为落叶层 >林冠层 >土壤层 .4个月后 ,不同放菌频度和放菌量区的带菌量基本一致 ,稳定在一定范围内波动 .在 12个放菌方案中 ,1年 2次放菌、每次放菌37.5g·hm-2 (10 0 0× 10 8孢子·g-1)为最佳放菌方案 .

Annual dynamics of Beauveria bassiana in 12 experimental plots of masson pine plantation inoculated at different doses and frequencies was investigated.The results showed that inoculum density of B.bassiana in the plantation was correlative to released inoculum in initial stage after B.bassiana released .In this period, the amount of inoculum densities at different niches of the plantation was in order of litter layer>crown layer>soil layer. Four months later, inoculum densities in X plots (2 releases a year), Y plots (1 release a year) and Z plots (1 release every two years inoculated one year ago) fluctuated stably within certain ranges. Therefore, the optimal frequency and dosage of inoculation releasing B. bassiana was determined as twice a year, and 37.5g·hm -2 (10 11 conidia·g -1 ) each time.