细胞遗传学动态检测在慢性粒细胞白血病中的应用

The consecutive study of cytogenetic in chronic myelad leukemia

目的 :评价细胞遗传学动态改变在判断慢性粒细胞白血病 (CML)病程进展中的作用 ,以及干扰素对CML细胞遗传学缓解率的影响。方法 :对 2 0例CML患者进行动态细胞遗传学检测。结果 :2 0例患者初诊时均具有 9号和 2 2号染色体易位 ,形成Ph染色体 ,2 0例患者在监测过程中 ,有 5例患者出现克隆转化 ,此 5例患者均在短期内急变。在应用干扰素治疗的 12例患者中有 4例患者在治疗中曾转变为正常核型 ,达细胞遗传学完全缓解。结论 :应用常规细胞遗传学检查对于诊断临床上不典型的CML有重要作用。在CML病程中动态监测细胞遗传学改变 ,如果发生克隆转化 ,可以预测急变 ,以便提早治疗。早期应用干扰素治疗CML可以使Ph染色体转阴 ,监测其动态变化可以指导临床治疗。

Objective:To evaluate the cytogenetic changes in the course of CML.Method:Twenty CML patients were studied using short time culture of bone marrow cell and G-banding technique.Result:All of the twenty patients have t(9;22),forming Philadelphia chromosome.Through the consecutive detection,karyotype evaluation exist in five patients.All of the five patients progressed to blast crisis in short time. We also detected four other patients' Philadelphia chronosome dispeared during the treatment using interforn.Conclusion:Our study suggest cytogeynetic analysis is important for the diagnosis of CML, especial for those whose symptoms is atypical. Through consective study, we can detect the karytype evaluation, which can forcase disease progressing. Using interferon in the early period, some patients Ph chronosome may dispear.