摘要
研究了低能离子注入 2 酮基 L 古龙酸 (2 KLG)生产菌的生物学效应。结果表明能获得较高的突变率和较广的突变谱。比较了三种离子注入 2 KLG生产菌的差异 ,确定了最佳诱变剂量和最适注入离子。由此筛选出若干高产突变株 ,其糖酸克分子转化率高达 97% ,目前已通过工业化生产实验 ,取得了显著的经济效益。
A new technology--low energy ion implantation and its mechanism and effects have been studied. We research into the mutagenic effects of 2 KLG strains implanted by low energy ion. The results show it can achieve higher rate of mutation and wider mutational spectrum. Compared with differences among 2 KLG strains implanted by H+, N+, Ar+, the best mutation dose was determined. Several high yield strains have been bred, their conversion rates from L sorbose to 2 KLG reached about 97%. This achievement has been applied to the industrial production.
出处
《高技术通讯》
EI
CAS
CSCD
2002年第11期41-46,共6页
Chinese High Technology Letters
基金
国家"九五"重点攻关资助项目
