脑室注射6-羟多巴胺对达乌尔黄鼠体温及体温调节能力的影响

Effects of Intraventricular Injection of 6-hydroxydopamine on the Body Temperature and Thermoregulation in the Ground Squirrel

观察了脑室注射6-OHDA对达乌尔黄鼠体温及体温调节能力的影响。结果表明:(1)脑室注射200μg 6-OHDA产生一个短暂的急性低体温效应,在室温环境中体温下降幅度为5℃左右,在5℃寒冷环境中体温下降7℃左右。脑室注射第二个剂量6-OHDA所引起的低体温效应比第一次明显减弱;中枢投入α-受体阻滞剂可以取消此低体温效应。(2)脑室注射6-OHDA对体温调节能力仅有暂时性影响,注药20小时后,体温调节能力即可恢复,动物在寒冷环境中和正常动物一样能够维持恒定的体温。这些观察结果为应用6-OHDA于冬眠实验工作提供了一个基本的参考依据。

The effects of intraventricular injection of 6-hydroxydopamine (6-OHDA) on the body temperature and thermoregulation were observed in the ground squirrel (Citellus dauricus). The results showed: (1) i. c. v. administration of 6-OHDA (200μg) was found to cause a mild (5℃) reduction in body temperature at room temperature, a greater fall (7℃) in 5℃ cold environment. The hypothermia recovered about 7 hours after administration of 6-OHDA at room temperature. (2) The acute hypothermia effect of 6-OHDA was abolished by central pre-administration of phentolamine. This blockade suggested that the acute hypothermia was the result of endogenous catecholamine release after administration of 6-OHDA. (3) Despite the marked actue thermal response to 6-OHDA, there appers to be no long-lasting effect on the temperature regulatory center. The ground squirrels treated with 6-OHDA were able to keep constant body temperature in cold environment 20 hours after 6-OHDA administration.