硒对糖尿病大鼠血糖及脂质代谢影响的实验研究

Study on Effects of Selenium on Blood Glucose and Lipid Metabolism of Diabetic Rats

目的 观察硒对四氧嘧啶诱导的糖尿病大鼠糖、脂代谢的影响并探讨其相关生化机制。方法 健康雄性Wistar大鼠 32只 ,随机分成 3组 :正常对照组、阳性对照组、补硒组。补硒组每日以 5 0 μg/kg·bw的硒灌胃。 6 0天以后 ,进行相关指标的生化检测。结果 补硒组大鼠血糖水平较实验前明显降低 ,糖耐量得到相应改善 ;TC、TG、LDL C、AI水平明显低于阳性对照组 ,HDL C高于阳性对照组 ;红细胞SOD、全血GPX活力明显提高 ,MDA水平下降。结论 硒通过提高糖尿病大鼠体内的抗氧化能力在一定程度上改善了其糖。

Objective To observe the effects of selenium on glucose and lipid metabolism in diabetic rats,and its related biochemical mechanism.Method Thirty two healthy male Wistar rats were assigned to three groups (normal control,diabetic control,and selenium supplemented group).Fifty μg/kg·bw was supplemented to selenium supplemented group each day by gavage for 60 days.At the end of the treatment,the blood biochemical analysis was performed.Results Blood glucose in selenium supplemented group decreased significantly than before experiment,and glucose tolerance was improved relevantly.The levels of serum TG,TC,LDL C and AI in selenium supplemented group were lower than those of diabetic control,while the serum HDL C levels were higher.The activity of SOD in erythrocytes and blood GPX increased and the content of MDA decreased obviously in selenium supplemented group.Conclusion Selenium could partially improve the disorders of glucose and lipid metabolism by means of increasing the antioxidant activity in diabetic rats.