流动注射化学发光增强法测定健民咽喉片中的没食子酸

Flow Injection Analysis of Gallic Acid in Jianmin Yanhou Tablets with Enhanced Chemiluminescent Detection

在pH 9.4的Na2 CO3 NaHCO3介质中 ,没食子酸对鲁米诺 K3Fe(CN) 6 发光体系有很强的增敏作用。据此建立了流动注射化学发光增强法测定没食子酸的方法。发光信号的增强值 (ΔI)与没食子酸的浓度在1 0× 1 0 - 6 ～ 8.0× 1 0 - 9mol L的范围内呈良好的线性关系 ,检出限为 5 .6× 1 0 - 9mol L。对于 1 .0× 1 0 - 7mol L的没食子酸测定 ,相对标准偏差为 2 .2 % (n =1 1 )。

A novel flow injection procedure was developed for the determination of gallic acid in Jianmin Yanhou tablets based on the enhancement effect of gallic acid on the chemiluminescence of luminol～K 3Fe(CN) 6 system. The method has the merits of higher sensitivity, higher selectivity, wider linear range, simpler instrumentation. It is applicable for the determination of gallic acid in range of 8.0×10 -9 mol/L～1.0×10 -6 mol/L with a detection limit of 5.6×10 -9 mol/L. The relative standard deviation is 2.2% for the determination of 1.0×10 -7 mol/L gallic acid (n=11). The method has been sucessfully used to determine gallic acid in Jianmin Yanhou tablets.