脂肪酶抑制剂产生菌筛选

Screening of lipase inhibitor producing strain

为了开发减肥用品的微生物菌种 ,用脂肪酶活性为筛选模型分别以肉汤培养基和马铃薯培养基培养土壤分离获得 1 1个菌株LA、LB、LC、LD、LE、LF、LG、MA、MB、MC和MD ,且同时考察了各菌株发酵液对淀粉酶、蛋白酶的抑制效果 ,结果表明LC、LE、LF、LG、MC和MD对淀粉酶有抑制作用 ,其抑制率分别为3 7%、5 8%、32 %、3 2 %、70 2 %和 73 5 % ;LA、LB、LC、MB对蛋白酶有抑制效果 ,抑制率分别为 55 %、31 2 5 %、37 5 %和 2 7 3 % ;LA、LB、LC、LD、LE、LF、MC和MD对脂肪酶有抑制效果 ,抑制率分别为 79%、54 8%、2 0 9%、1 2 9%、61 3 %、80 6 %、2 4 7%和 35 1 %。菌种LF对脂肪酶有较强的抑制作用 ,对淀粉酶抑制作用较小 ,对蛋白酶没有抑制作用。初步鉴定该菌属于芽孢杆菌属 ,命名为BacillusspLF。

To develop anti obesity products,a screening method using the inhibitory activity of lipase as model was established. Strains LA, LB, LC, LD, LE, LF, LG, MA, MB, MC, MD isolated from soil were cultured. The inhibitory activities of their broth on amylase, protease and lipase were determined. Results showed that LC, LE, LF, LG, MC and MD could inhibit amylase by 3 7%, 5 8%, 32%, 3 2%, 70 2% and 73 5% respectively; LA, LB, LC and MB could inbibit protease by 55%, 31 25%, 37 5% and 27 2% respectively; LA, LB, LC, LD, LE, LF, MC and MD could inhibit lipase by 79%, 54 8%, 20 9%, 12 9%, 61 3%, 80 6%, 24 7% and 35 1% respectively. The results suggest that strain LF showed high lipase inhibitory activity while low amylase inhibitory activity and no protease inhibitory activity. Strain LF is designated as Bacillus sp.