帕金森病大鼠颈动脉体球细胞移植后TH、GFAP和TNF-α表达的动态变化

The dynamic changes of the expression of TH, GFAP and TNF-α in glomus cell of carotid body grafts in a rat model of Parkinsons disease

目的 探讨帕金森病 (PD)大鼠颈动脉体球细胞移植后细胞存活状况和宿主的反应。方法 立体定位注射 6 羟多巴胺 (6 OHDA)制备偏侧PD大鼠模型 ,右侧纹状体内分别移植入自、异体颈动脉体和胚胎大鼠中脑组织块 ,移植后 2、4、8和 12周用免疫组织化学法同步检测酪氨酸羟化酶(TH)阳性细胞数、移植区周围胶质纤维酸性蛋白 (GFAP)和肿瘤坏死因子 α(TNF α)表达水平的改变。结果 移植后 12周与胚胎中脑组织移植组比较 ,自、异体颈动脉体球细胞移植组存活TH+细胞显著增多 (P <0 .0 5 ) ,但自、异体颈动脉体球细胞移植组间比较差异无显著性。各移植组移植后 2周 ,移植区内GFAP和TNF α阳性数显著增高 ,移植后 12周时下降 ,但仍高于健侧 ,差异仍有极显著性 (P <0 .0 1)。结论 颈动脉体球细胞块移植治疗可显著提高TH+细胞存活率 ,而移植区GFAP和TNF

Objective To study the survival status, expression of glial fabrillary acidic protein (GFAP) and tumor necrosis factor-α (TNF-α ) of glomus cells within carotid body grafts in a hemiparkinsonian rat model. Methods Rats with unilateral 6-hydroxydopamine (6-OHDA) lesions of the right dopaminergic neurons of substantia nigra received intrastriatal graft of homo-or hero-intrastriatal glomus cells within carotid body (group A) and fetal mesencephalic neurons (group B) respectively. The cell number positive for tyrosine hydroxylase (TH +) and the changes in the expression levels of GFAP and TNF-α were analyzed at week 2, 4, 8 and 12 after grafting respectively. Results The alive neurons positive for TH were significantly increased at 12th week in group A as compared with those in the group B (P< 0.05), but no significant difference was found within group A. In each group 2 weeks after grafting, the cell number positive for GFAP and TNF-a was significantly increased, then decreased at 12?th week, but also significantly higher than in the intact side, respectively (P< 0.01). Conclusion The intrastriatal graft of glomus cells within carotid body in rats with 6-OHDA lesions could elevate the survival rate of TH +, but the GFAP and TNF-α expression had a negative effect on it.