一氧化氮与妊娠早期人巨细胞病毒宫内活动性感染的关系

Relationship of nitric oxide with intrauterine human cytomegalovirus active infection during early pregnancy

目的 探讨一氧化氮与妊娠早期人巨细胞病毒 (HCMV)宫内活动性感染的关系。方法 选取既往有自然流产、死胎、胎儿畸形、胎儿生长受限等异常妊娠史并行人工流产术的早孕妇女 81例 ,应用逆转录 聚合酶链反应技术检测其胎盘绒毛组织HCMV mRNA的表达 ;酶联免疫吸附试验法测定其血清HCMV IgM ;原位杂交法检测其蜕膜巨噬细胞诱生型一氧化氮合酶 (iNOS) mRNA的表达并行定量分析。结果 (1) 81例孕妇中有 6例胎盘绒毛组织HCMV mRNA阳性 ,14例血清HCMV IgM阳性 ,阳性率为 17 3 % (14/ 81)。依据HCMV mRNA与HCMV IgM检测结果 ,81例孕妇分为 3组 :Ⅰ组 :HCMV mRNA与HCMV IgM均阳性 5例 ,其宫内传播率为 3 5 7% (5 / 14) ;Ⅱ组 :HCMV mRNA阴性而HCMV IgM阳性 9例 ;Ⅲ组 :HCMV mRNA与HCMV IgM均阴性 66例。另有 1例HCMV IgM阴性者其胎盘绒毛组织HCMV mRNA阳性。 (2 )Ⅰ组患者蜕膜iNOS mRNA呈强表达 ,其吸光度值为 0 412±0 0 19,明显高于Ⅱ组的 0 172± 0 0 3 3与Ⅲ组的 0 167± 0 0 3 3 ,Ⅰ组与Ⅱ组及Ⅲ组比较 ,差异有显著性(P <0 0 5 ) ;Ⅱ组与Ⅲ组比较 ,差异无显著性 (P >0 0 5 )。 1例HCMV IgM阴性而胎盘绒毛组织HCMV mRNA阳性者 ,蜕膜iNOS mRNA也呈强表达 ,其吸光度值为 0 40 3。结论 一氧化氮可?

Objective To study the relationship of nitric oxide (NO) with intrauterine human cytomegalovirus (HCMV) active infection during early pregnancy Methods Reverse trancscript polymerase chain reaction (RT PCR) was used to screen HCMV mRNA of chorionic villi during early pregnancy in 81 cases who had poor pregnant history The serum HCMV IgM was measured by enzyme linked immunoadsorbent assay (ELISA) and the expression of decidual iNOS mRNA was investigated with in situ hybridization (ISH) Results (1)Six cases were HCMV mRNA positive in chorionic villous tissues and 14 cases with serum HCMV IgM positive among cases, the positive rate of serum HCMV IgM was 17 3%(14/81) All cases were divided into three groups according to HCMV mRNA and serum HCMV IgM Group Ⅰ: 5 cases with HCMV mRNA positive and IgM positive,and the transmission rate between pregnant women and fetus was 35 7%(5/14); Group Ⅱ: 9 cases with HCMV mRNA negative and IgM positive, Group Ⅲ: 66 cases with HCMV mRNA and IgM negative; In addition, 1 case with HCMV mRNA positive but IgM negative (2) The expressive quantity of decidual iNOS mRNA in group Ⅰ was significantly higher than those in group Ⅱ and Ⅲ ( P <0 05) The case with HCMV mRNA positive and serum IgM negative also has a strong staining of decidual iNOS mRNA Conclusions NO may play a critical role in intrauterine HCMV active infection, NO could be served as an assistant marker of HCMV infection