组织内微量抗原的催化信号扩增法检测

Detection of trace antigens in tissues by catalyzed signal amplification

目的 探讨催化信号扩增法 (catalyzedsignalampli fication ,CSA)在检测组织内微量抗原中的作用及意义。方法 分别应用免疫组织化学ABC法和CSA法 ,对 5 4例霍奇金淋巴瘤 (Hodgkin’slymphoma ,HL)和 1例人巨细胞病毒 (humancytomegalovirus,HCMV)感染的对照涎腺组织中 ,HCMV的立即早期抗原DDG9、早期抗原CCH2和低基质蛋白抗原AAC10进行检测 ,并比较阳性信号的强弱及阳性率。结果 应用ABC法 ,仅在 1例对照组织中检测到DDG9、CCH2及AAC10的弱阳性信号 ,在 5 4例HL中 ,未检测到任何阳性信号 ;而应用CSA法不仅对照组织中DDG9、CCH2及AAC10均呈强阳性 ,而且有 6例 (11.1% )HLDDG9/CCH2呈阳性 ,5例 (9.3% )HLAAC10呈阳性。结论 CSA是一种较ABC法敏感的免疫组织化学染色方法 。

Aim To explore the significance of catalyzed signal amplification (CSA) in detection of trace antigens in tissues. Methods The immunohistochemical staining of ABC and CSA were adopted and compared in detecting DDG9, CCH2 and AAC10 of human cytomegalovirus (HCMV) in 54 cases of Hodgkin's lymphoma (HL) and 1 case of salivary tissue with HCMV infection. Results By ABC method, only weakly positive signals of DDG9, CCH2 and AAC10 were detected in salivary tissue and none was positive in HL tissues. However, using CSA method, DDG9 and CCH2 in 6 cases and AAC10 in 5 cases were positive in 54 cases of HL, while DDG9, CCH2 and AAC10 were strongly positive in salivary tissue. Conclusion CSA method is a more sensitive immunohistochemical staining compared with ABC method, and it can be used to detect trace antigens in the tissues which can not be detected by ABC method.