血管紧张素原M235T基因多态性与重庆地区原发性高血压的关系

Relationship between angiotensinogen gene M235T polymorphism and essential hypertension in Chongqing

目的 研究血管紧张素原M2 35T基因多态性与重庆地区人群原发性高血压的关系。方法 采用聚合酶链反应结合限制性内切酶消化 ,对重庆地区 92例原发性高血压患者和 80例血压正常者血管紧张素原的M 2 35T基因多态性进行检测。结果 原发性高血压组TT基因型频率为 6 4 .1% ,明显高于对照组 (P <0 .0 5 )。相对于MM基因型 ,暴露于TT基因型的OR值为3.835 (P <0 .0 5 )。原发性高血压组T等位基因频率为 79.3% ,明显高于对照组 (P <0 .0 1)。相对于M等位基因 ,T等位基因的OR值为 1.90 3(P <0 .0 1) ,随着等位基因T的增加 ,患高血压的危险性也增加。结论 血管紧张素原M2 35T基因多态性与重庆地区人群原发性高血压密切相关 ,TT基因型可能是重庆地区原发性高血压的易感基因型。

Objective To investigate the association between M235T allele polymorphism in exon 2 of angiotensinogen (AGT) gene and essential hypertension(EH). Method Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene polymorphism in 80 normotensive controls and 92 hypertensive patients. Result The frequencies of T allele and TT genotype were significantly higher in hypertensive patients than in normotensive controls( 79.3% vs 66.9%;64.1% vs 50%,respectively ). The odds ratio exposed to TT genotype was 3.835 comparing with MM genotype (P<0.05). It was suggested that TT genotype be the susceptible genotype to EH in Chongqing. The odds ratio exposed to T allele was 1.903 comparing with M allele (P<0.01). That means that the risk of EH has increased with the T allele frequency increasing.Conclusion AGT M235T gene polymorphism is closely associated with essential hypertension in Chongqing. TT genotype may be susceptible genotype to EH in Chongqing.