草莓果实成熟衰老与Ca^(2+)、CaM、Ca^(2+)-ATPase和活性氧代谢的关系

Relationship Between Maturation, Senescence and Ca^(2+),CaM Content, Ca^(2+)-ATPase Activity, and Active Oxygen Metabolism in Strawberry Fruits

研究了春星草莓果实成熟衰老过程中Ca2 + 、CaM含量、Ca2 + ATPase活性和活性氧代谢的变化 ,结果表明 ,随果实成熟衰老 ,可溶性Ca2 + 含量和SOD活性呈下降趋势 ,O2-·产生速率呈升高趋势 ,Ca2 + ATPase活性先达到高峰后降低。CaM含量以采前白熟期和采后明显衰老期为最高。与常温 (2 5℃ )贮藏相比 ,低温 (4℃ )贮藏中上述生化变化较为缓慢。表明细胞内钙信使系统的活化和活性氧自由基的积累与果实成熟衰老密切相关。

Changes of the content of Ca 2+ and CaM, Ca 2+ ATPase activity and active oxygen metabolism during strawberry (cv. Chunxing) fruits maturation and senescence were investigated. The results showed that the soluble Ca 2+ content and SOD activity in fruits decreased and O 2 -· production rate increased, the Ca 2+ ATPase activity peaked at first and then declined during fruits maturation and senescence. There were highest CaM content at white stage in preharvest fruits and at marked senescence stage in postharvest ones. The above biochemical changes in fruits stored at low temperature(4℃)were slower than those stored at normal temperature(25℃). Thus, it indicated that the stimulation of calcium messenger system and accumulation of active oxygen free radical were closely related to fruits maturation and senescence.