低能量红激光局部照射对自体移植静脉内膜增生的影响

The Role of Low Power Red Laser Illumination on Neointimal Formation in Vein Graft Model

目的 探讨低能量红激光局部照射对自体移植静脉内膜增生的影响。方法 取 80只大鼠 ,随机分为单纯自体静脉移植组 (对照组 ,n =40 )和低能量红激光局部照射组 (激光组 ,n =40 )。两组大鼠分别于术后第 3d、7d、14d及2 8d取移植静脉段 (每时相组各 10只 )固定 ,行HE染色、弹力纤维染色及增殖细胞核抗原 (PCNA)免疫组化染色 ,应用光学显微镜和计算机图像分析系统分析测定其内膜厚度和管腔面积。结果 术后第 3d ,两组管腔横截面积及内膜平均厚度差异无显著性意义 (P>0 .0 5 ) ;激光组术后第 7d、14d、2 8d内膜平均厚度低于对照组 (P<0 .0 5 ) ,管腔横截面积明显大于对照组 (P<0 .0 5 ) ;PCNA免疫组化染色结果显示 ,激光组移植静脉平滑肌细胞 (VSMC)增殖受到明显抑制 (P<0 .0 1)。结论 低能量红激光局部照射可以抑制自体移植静脉内膜及VSMC的增生 ,减少再狭窄的发生。

Objective To evaluate the effect of low power red laser illumination on the intimal proliferative response in vein graft models.Methods Autogenous vein graft models were established in 80 rats by transplanting jugular vein to carotid artery by end to end anastomosis, and were randomized into two groups: control group (graft nonilluminated), laser illumination group (0.9 J/cm 2).The grafted veins were harvested at 3,7,14 or 28 day respectively after operation. IH (intimal hyperplasia) and SMC (smooth muscle cell) proliferations were pathologically and immunohistochemically observed and analyzed by computer digitizing system. Results There were no significant differences in the intimal average thickness and the areas of lumen between two groups for 3 day. Laser group was significantly lower than the control in both the intimal average thickness and the stenosis of lumen at 7 day,14 day and 28 day ( P <0.05).Immunohistochemical analysis of PCNA indicate the decreased positive cell in laser group compared with the control group ( P < 0.01) .Conclusion These preliminary results demonstrate that a certain density of low power red laser illumination in vein graft inhibits SMC proliferation and neointimal hyperplasia in rat.