肿瘤坏死因子-α和细胞间黏附分子-1在大鼠实验性脑震荡脑组织中的表达研究

Expressions of tumor necrosis factor-α and intercellular adhesion molecule-1 in rat cerebral concussion

目的 :探讨大鼠实验性脑震荡脑组织中肿瘤坏死因子 α(TNFα)和细胞间黏附分子 1(ICAM 1)的表达及其意义。方法 :采用 Wistar雄性二级大鼠复制脑震荡动物模型 ,于伤后 1、3、7、14及 30日活杀取脑组织 ,经免疫组织化学等技术研究脑震荡发生、发展中 TNFα和 ICAM 1的变化规律。结果 :砝码 10 0 g组见典型脑震荡的表现 ,其病理改变为脑血管收缩与扩张 ,脑组织淤血与水肿 ,神经元变性、凋亡与坏死。 TNFα于伤后 1日表达增强 ,阳性部位见于大脑皮质、海马神经细胞胞浆中 ,伤后 3日达高峰 ,7日逐渐减少 ,14日后基本恢复至正常水平。 ICAM 1于伤后 1日 ,见海马、大脑及小脑的血管内皮细胞和血管壁明显增多 ;此外海马及大脑皮质神经元亦见阳性 ,于 7日达高峰 ,14日后开始减少 ,30日仍高于正常水平。结论 :脑震荡以血液循环障碍及实质细胞变性和坏死为主要病理改变 ;TNFα参与脑震荡发生时早期炎症反应、血管损伤及血脑脊液屏障功能的调节 ;ICAM 1参与脑震荡发生时炎症反应、血管内皮细胞黏附及神经细胞损害的过程。

Objective:To study the significance of changes in tumor necrosis factorα(TNFα) and intercellular adhesion molecule1(ICAM1) in rat cerebral concussion.Methods:Eighty Wistar male rats were used for animal model of cerebral concussion,and animals were sacrificed on days 1,3,7,14 and 30 after injury and the brain tissue were taken off.The expressions of TNFα and ICAM1 were determined at various intervals of cerebral concussion by immunohistochemistry staining.Results:Rats in 100 g group were seen the clinical situation for typical cerebral concussion.The pathologic changes included the cerebral vascular constriction and dilatation,congestion and edema of cerebral tissue,and neuronal degeneration as well as necrosis. TNFα was increased on day 1,and the positive area was found in the cytoplasm of nervous cells in cerebral cortex and hippocampus,peaking on day 3,decreasing on day 7 and tending to normal range on day 14. ICAM1 was increased on day 1,and the positive area was observed in the cytoplasm of endothelial cells and nervous cells in hippocampus,cerebral cortex and cerebellum,peaking on day 7,decreasing on day 14 and maintaining on day 30.Conclusions:The main pathologic changes of cerebral concussion are circulatory disorder and nervous cells degeneration,apoptosis as well as necrosis.TNFα may participate in the development of early inflammatory,blood vessel damage and regulation of bloodbrain barrier in cerebral concussion. ICAM1 may play an important role in the pathogenesis of inflammatory response,endothelial cell adhesion of blood vessel and the damage of nervous cells.