卷枝毛霉3.2208条件优化产类可可脂的研究

Study on Covoa Butter Equivalent Production by Mucor Circinelloides 3.2208 in Optimum Condition

通过正交实验,得出卷枝毛霉3.2208产类可可脂的最佳培养条件为:接种量0.5ml;温度33℃;pH值,6.0;C/N比,100;培养时间6d;氮源为蛋白胨;碳源为麦芽糖;FeSO4·7H2O0.01g/L;ZnSO4·7H2O0.01g/L;MgSO4·7H2O0.0005g/L;MgSO4·7H2O0.8g/L;油酸0g/L。在该条件下,卷枝毛霉的CBE得率为3.61%,油脂系数为0.29,其二位不饱和脂肪酸为79.08%。

Through the orthogonality experiment, the optimization culture condition for accumulating CBE by Mucor circinelloides 3.2208 was achieved as following:inoculum quantity 0.5ml;temperature 33℃;pH6.0;C/N ratio100;culture time 6 days;nitrogen source is peptone;carbon source is maltose;FeSO4·7H2O 0.01g/L;ZnSO4·7H2O 0.01g/L;MnSO4·4H2O 0.0005g/L;MgSO4·7H2O 0.8/L;Oleic 0g/L.Under such culture condition,we gain the Mucor circinelloides 3.2208 CBE 3.61%from dry cell.The lipid coefficient is 0.29,the Sn-2unsaturated fatty acid is up to 78.08%.