α-乙酰乳酸脱羧酶高产菌株的选育及产酶条件的研究

Enzyme Breeding and Producing Conditions of α-Acetylactate Decarboxylase Strain

以从土壤中分离出的9株地衣芽孢杆菌株,通过产酶实验发现BL-4的产酶活性最好,通过UV和NTC对BL-4进行复合诱变,获得了一株产酶活性比出发菌株BL-4高1.22倍的α-乙酰乳酸脱羧酶高产菌株,并研究了该菌株的最佳发酵产酶条件:培养温度30℃,发酵时间36h,发酵液起始pH6.5。

A high yield α-acetylactate decarboxylase producing strain was obtained by selecting and compound mutagenesis of UV and NTG with 9 soil Bacillus Lichenforms as starting strain.Among 9 native strains,BL-4 had the highest production of α-acetylactate decarboxylase.After UV mutagenesis of BL-4,the production of α-acetylactate decarboxylase of selected mutated strain(BL-4-UV-3) increased 43.3%.Then BL-4-UV-3 took place the second mutagenesis under NTG,the production of α-acetylactate decarboxylase of selected mutated strain (BL-4-UV-3-NTG-11) increased 1.22 times compared to native strain BL-4.The optimum enzyme producing conditions were 30℃,initial pH at 6.5,and 36h fermentation.