维生素C对大鼠肾结石模型体内活性氧及成石的影响

Effect of vitamin C on reactive oxygen species and their relation to formation of calcium oxalate nephrolithiasis in Wistar rat models

目的 观察不同剂量维生素C(VitC)对大鼠肾结石模型体内活性氧 (ROS)的影响及VitC、ROS与肾结石形成的关系。 方法 用乙二醇诱导Wistar大鼠产生肾草酸钙结石。 30只大鼠分为正常组 ,成石组 ,治疗组 (分三个不同剂量 ,VitC 2 5、10 0、4 0 0mg·kg-1·d-1) ,喂养 15d。测定血和右肾组织中丙二醛 (MDA)、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶(GSH Px)含量 ;左肾冰冻切片 ,偏光显微镜观察结晶情况。 结果 成石组血和组织中的MDA分别为 (43.89± 5 .10 )nmol/ml和 (6 .2 0± 2 .0 0 )nmol/gpr,较正常组增高 ,差别有显著性意义 (P <0 .0 1和P <0 .0 0 1) ,而抗氧化酶显著降低 (P <0 .0 1和P <0 .0 0 1)。VitC 4 0 0mg·kg-1·d-1治疗组血MDA(30 .80± 4 .6 9)nmol/ml,较成石组降低 (P <0 .0 5 ) ,与正常组比较差别无显著性意义 (P >0 .0 5 ) ,血SOD较成石组降低 (P <0 .0 5 ) ,血CAT、GSH Px较成石组升高 (P <0 .0 1) ;VitC 4 0 0mg·kg-1·d-1治疗组肾组织中MDA(11.96± 2 .4 4 )nmol/gpr ,较成石组升高 (P <0 .0 5 ) ,SOD较成石组升高 (P <0 .0 5 ) ,CAT、GSH Px较成石组有所降低。肾组织晶体形成与VitC剂量呈正相关 (r =0 .6 6 85 ,P <0 .0 1)。 结论 高草酸尿可使机体活性?

Objective To determine the effect of vitamin C on reactive oxygen species(ROS) and their relation to formation of nephrolithiasis in Wistar rat models. Methods Hyperoxaluria and CaOx crystal were produced in Wistar rats by ethylene glycol in drinking water.The animals were randomly divided into 3 groups,ie,control group,nephrolithiasis group and vitamin C treatment groups at a dose of 25,100 and 400 mg·kg -1 ·d -1 .After 15 days,malondialdehyde (MDA),catalase (CAT),superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) in blood and kidney tissue were measured.The presence of CaOx crystal were scored on a basis of -～++++. Results MDA increased and antioxidase decreased significantly in kidney tissue and blood of the nephrolithiasis group.Compared with the nephrolithiasis group,MDA (30.80±4.69)nmol/L and SOD decreased significantly ( P < 0.05 ) in blood,but MDA (11.96±2.44)nmol/g pr and SOD increased significantly in kidney tissue of the vitamin C treatment groups ( P < 0.05 ).The forming of CaOx crystal had a positive correlation with the dose of vitamin C ( r=0.6685,P < 0.01 ). Conclusions Hyperoxaluria can produce more ROS in mammals.The lipid peroxidation may be the cause of the formation of calcium oxalate nephrolithiasis. The alteration of ROS and the formation of nephrolithiasis have relation with the dose of vitamin C.