双标记人肝癌裸鼠实验研究

STUDY OF HUMAN PRIMARY HEPATOCARCINOMA IN NUDE MICE SECRETING AFP & HBsAG

应用简易实验室和层流架成功地繁育SPF级裸鼠,并建立起双标记—AFP和HBsAg^+的人肝癌裸鼠模型。移植瘤已传5代,生长稳定,潜伏期缩短;经组织病理学、超微结构病理学和免疫组织化学研究,确认移植瘤来自于人原发性肝癌。应用免疫试剂盒和电子显微镜技术进一步证实荷瘤小鼠移植瘤不仅分泌AFP,而且还分泌。HBsAg,瘤组织含有HBsAg蛋白纤丝样特征的细胞。一种适合于癌细胞存活的转运培养液初步用于肝癌的取材,更深入的工作有待于进一步开展。

The hetrotransplanted human hepatocellular carcinoma (HCC) secreting both AFP &-HBsAg was first established in Fujian province. The nude mice bearing tumor grew well and kept up 5th passage. Under histological examination, supported by electron microscopy and immunohistochemistry, the transplanted tumor retained approximately the morphological characteristics of original tumor. Shown by TEM, the transplanted tumor cells displayed some figures seen in normal liver cells, such as bile canilicull, junctionai complexes which were incomplete. HBsAg-like crystal structure was seen within RER and SER in some tumor cells. It is suggested that the close relationship between HBV and HCC be presented.It is reasonable that the development of double marked tumor in nude mice is an-useful animal model for the studies of cell biology, chemosensitivity and immunotherapy in HCC. A transfer solution, which is suitable for the survival of tumor cells, was presented in this paper. Owing to the limited passages of nude mice, the further efforts will andoubtedly be directed toward the schedule of biochemistry, growth dynamics and chromosome characteristics in hetrotransplanted HCC.