人食管癌裸鼠移植瘤株建立及其应用的研究

ESTABLISHMENT OF TRANSPLANTABLE MODELS OF HUMAN ESOPHAGEAL CARCINOMA IN NUDE MICE AND THEIR THERAPEUTIC CHARACTERISTICS

用人食管癌组织直接接种于裸鼠,建立两株裸鼠移植瘤株,分别命名为人食管鳞状细胞癌裸鼠移植瘤(HEC_2)和人食管腺鳞癌裸鼠移植瘤(HEC_6),两瘤株的鼠间传代达40余代,历时3年,生长稳定,潜伏期短,移值成功率达100％。并应用于抗癌药物和放射增敏的实验研究,结果表明:平阳霉素对HEC_6有明显的抑制作用,重复两次,抑制率分别为56.5％和8.87％;血卟啉衍生物(HPD)对HEC_2 X线分次照射有明显增敏作用。

We have successfully established two human esophageal carcinoma models in nude mice by transplantation of surgical specimen from patients with esophageal cancer Histopathological study revealed that one of these to be squamous call carcinoma (HEC_2) and the other adeno-squamous cell carcinoma (HEC_6). Light and electron microscopy showed that they were similar to that of the original human tumors. The transplanted tumors have been maintained for more than three years through more than 40 passages. The tumors grew steadily and fast. The rate of inoculation was 100%. Bleomycin A5 and 5-Fluorouracil (5-Fu) were used for treatment of HEC_6. Results showed that Bleomycin A5 exhibited a remarkable growth inhibition on the HEC_6. The inhibitory rates of two separate experiments were 56.5% and 87.8% respectively comparing with the control group (P<0.01). The inhibitory rates of 5-Fu were 6.5% and 36.6% respectively, there was no statistic difference compared to the control group. HEC_2 was treated by X-ray or X-ray combined with Hematoporphyrin derivative (HPD), the tumor inhibitory rates were 57.7% and 85.4% respectively The results showed that combination of HPD with orthovoltage X-ray irradiation exhibited enhanced effect, and the result was repeatble.