摘要
目的 观察人类野生型p5 3 (wtp5 3 )基因对人胰腺癌细胞系的抑制作用。方法 用逆转录病毒为载体将外源性wtp5 3基因导入人胰腺癌细胞系PC -2 ,通过体外及小鼠体内实验研究转入基因的表达及对肿瘤的抑制作用。结果 p5 3在转染细胞PC -2 /p5 3中表达水平提高。外源性wtp5 3基因的导入和表达能使PC -2细胞的生长速率减低 ,软琼脂集落形成能力下降 ,G0 +G1期细胞比例增加 ,凋亡指数升高 ,裸鼠体内成瘤能力明显下降。结论 逆转录病毒载体介导的外源性野生型p5
Objective To observe the inhibitory effects of human wild-type p53(wtp53) gene on human pancreatic cancer cell line. Methods Retroviral vector was used to introduce exogenous wtp53 gene into human pancreatic cancer cell line PC-2, Gene expression and tumor inhibition were studied in vitro and in vivo . Results The expression of p53 in transfected cell lines(PC-2/p53) increased. The introduction and expression of exogenous wtp53 gene resulted in decreased growth rate and reduced ability to form colony in soft agar in PC-2 cells. The G 0+G 1 ratio increased and S ratio decreased in cell cycle distribution, and apoptosis index significantly increased in PC-2/p53 cells. The tumorigenicity of PC-2/p53 cells in nude mice was significantly suppressed by exogenous p53. Conclusion Exogenous wtp53 mediated by retroviral vector can inhibit the growth of human pancreatic cancer cell.
出处
《广东医学》
CAS
CSCD
2002年第11期1141-1143,共3页
Guangdong Medical Journal