肾毒血清性肾炎大鼠肾组织中核因子-κB活化及单核细胞趋化蛋白-1表达

Activation of Nuclear Factor-kappa B and the Expression of Monocyte Chemoattractant Protein-1 in Experimental Glomerulonephritis of Rats

目的:探讨核因子-κB(NF-κB)活化在肾毒血清性肾炎发病机制中的作用。方法:肾毒血清肾炎应用兔抗鼠肾小球基底膜肾毒血清制备。应用凝胶电泳迁移率检测肾组织中NF-κB活化;采用免疫组化及彩色病理图文分析系统观察肾小球及肾小管中单核细胞趋化蛋白-1(MCP-1)表达,并分析其与蛋白尿和肾小球细胞数之间的关系。结果:模型组肾组织中NF-κB活化较正常对照组显著上调;肾小球及肾小管中MCP-1表达分别为(24.37±7.06)个/肾小球横切面(gcs)和(54.78±11.49)%,较正常对照组显著升高(P<0.01),肾组织中NF-κB活化和MCP-1表达与单核细胞浸润和蛋白尿密切相关。结论:NF-κB活化在肾小球肾炎发病机制中发挥重要作用。

Objective: To investigate the action of nuclear factor kappa B (NF κB) in the pathogenesis of glomerulonephritis. Methods: Nephrotoxic sera nephritis (NTN) was induced by injecting anti GBM antibody into the tail veins of rats. NF κB activation was detected by Electrophoretic mobility shift assay (EMSA). The expression of monocyte chemoattractant protein 1 (MCP 1) in glomerulus and renal tubules of rats were assessed by immunochemistry and true color medical image system. Results: Significant up regulation of NF-κB activation was observed in NTN rats compared to control group, and so was in expression of MCP 1. NF κB activation and MCP 1 expression were associated with monocyte cell infiltration and the amounts of proteinuria. Conclusions: The activated NF κB may play a pivotal role in pathogenesis of glomerulonephritis.