牙周炎患者GCF及血清中IL-6测定及其与牙周炎的关系

The detection of IL-6 in gingival crevicular fluid and serum from the patients with periodontal disease

目的 研究龈沟液 (GCF)中白细胞介素 - 6 (IL - 6 )的来源及其与牙周炎的关系。方法 采用双抗夹心酶联免疫吸附法 (ELISA)测定了 72例成人牙周炎 ,13例牙龈炎 ,18例健康对照组GCFIL - 6含量 ,及 17例牙周炎患者 ,7例健康人血清IL - 6水平。结果 牙周炎患者GCFIL - 6水平显著高于健康人 ,牙周炎患者GCFIL- 6水平与牙周炎患者血清中IL - 6含量有显著性差异 ,牙龈炎患者血清IL - 6含量比正常对照组含量高 ,牙周炎患者GCFIL - 6含量与牙龈炎患者有显著性差异 ,重度牙周炎患者GCF中IL - 6含量高于轻度牙周炎患者 ,牙周炎患者GCF中IL - 6含量与出血指数 (BI)、牙周袋深度 (PD)、附着丧失 (AL)有正相关关系。结论 龈沟液中IL- 6主要来源于局部牙龈组织 ,IL - 6在牙周炎的发生。

Objective To study the source of IL-6 in gingival crevicular fluid(GCF) and the relationship between IL-6 and periodontal disease.Methods IL-6 levels in GCF were measured in 72 adult patients with periodontitis,13 patients with gingivitis and 18 health controls by sandwith enzyme-linked immunosorbent assays (ELISA).In addition,IL-6 level in serum was measured in 17 periodontitis and 7 healthy controls at the same time.Results IL-6 level in GCF of periodontitis was markedly higher than that of healthy controls.IL-6 level in GCF was much higher than that in serum of the patients with periodontitis.IL-6 level in GCF was higher in periodotitis than in gingivitis.IL-6 level in serum of gingivitis is higher than that of controls.IL-6 level in GCF is different between severe periodontitis and superficial one.Positive correlation was found between IL-6 level in GCF and BI?PD?AL.Conclusion IL-6 in GCF mainly derive from local gingival tissue,IL-6 plays a certain role in the course of origin and development of periodontitis.