恩诺沙星纳米乳对生长仔猪抗氧化GSH-ST和MDA影响

Effects of enrofloxacin nanoemulsions on antioxidant GSH-ST and MDA in growing pigs

试验选用恩诺沙星纳米乳制剂,进行生长仔猪的体内抗氧化性能试验。恩诺沙星纳米乳由恩诺沙星和十六烷基棕榈酸合成;纳米药物中的恩诺沙星粉剂含量5.0 mg/ml。其理化性能,纳米粒度实测平均粒径为(111.0±7.2)nm,分散指数为0.26±0.17,电动势为(-31.57±3.76)mv。拉丁方设计,恩诺沙星纳米乳分别按0、5.0、2.5 mg/kg和1.5 mg/kg剂量,口服给药后0、6、24 h与48 h时间前腔静脉采血。测定抗氧化指标丙二醛(MDA)和谷胱甘肽-S转移酶(GSH-ST)。试验结果,仔猪分别按0、1.5、2.5 mg/kg和5.0 mg/kg剂量口服给药后,6 h时间GSH-ST的检测值差异不显著(P>0.05);纳米粒药物为5.0 mg/kg时,GSH-ST达到了最高值为(39.52±18.41)mg/l。给药24 h和48 h,仔猪体内的GSH-ST检测值均出现显著性差异(P<0.05),说明组织细胞的抗氧化性能在发挥作用。机体内的MDA未出现显著差异(P>0.05),说明未对细胞造成损伤。

This experiment was conducted to study the effects of enrofloxacin nanoemulsions on antioxi-dant property of growing pigs. Enrofloxacin nanoemulsions was prepared using palmitic acids and enro-floxacin, which nanoparticle was(111.0±7.2) nm, polydispersity index was 0.26±0.17, electromotiveforce was(-31.57±3.76) mv and the drug of enrofloxacin was 5.0 mg/ml. A factorial design was usedin this experiment. Enrofloxacin nanoemulsions were taked to the pigs though mouth according to 0,5.0, 2.5 mg/kg and 1.5 mg/kg respectively, determinating the blood antioxidant index GSH-ST andMDA at 0, 6, 24 h and 48 h. The results show that enrofloxacin nanoemulsion with 0, 1.5, 2.5 mg/kgand 5.0 mg/kg in pigs, at 6 h GSH-ST has no significant difference(P>0.05), and bring a higher GSH-ST was(39.52±18.41) mg/l from the drug of 5.0 mg/kg. At 24 h and 48 h, significantly increased GSH-ST activity in blood(P<0.05), and had better effect on antioxidant property to cells in vivo. MDA hasno significant difference(P>0.05) in this experiment, however, no influence on the organs of the pigs.