一株降解呕吐毒素的青霉菌的分离与鉴定

Isolation and identification of a penicillium strain degraded deoxynivalenol

为了分离能降解呕吐毒素的菌株,试验以呕吐毒素为唯一碳源,对采集于洛阳市周围的农田、垃圾场、水沟边的土壤及瘤胃内容物等共55份样品进行初筛,然后用初筛的菌株来发酵粉碎的玉米秸秆以复筛呕吐毒素降解率较高的菌株,其中编号为Ma-1-4的菌株具有较强的降解呕吐毒素的能力,对玉米秸秆中呕吐毒素的降解率达46%。经形态学和r DNA-ITS序列同源性分析,确定该菌株为青霉属菌株。

In order to isolate the strains degraded deoxynivalenol, a total of 55 samples were collectedfrom the soil of the dump, farmland, gutter and the rumen contents surrounding the Luoyang, and thesamples were screened by using deoxynivalenol as the sole carbon source. The strains that could growin the medium with deoxynivalenol carbon source were detected for their deoxynivalenol-degrading abil-ity by adding to the pulverizing cornstalk. The strain Ma-1-4 showed good deoxynivalenol reduction ac-tivity, and the degradation rate could reach 46%. According to its morphological and playlogenetic anal-ysis of its r DNA-ITS sequence, the strain Ma-1-4 was preliminary identified to be penicillium.