利用响应面法体外优化复合酸化剂配方

Fomulation optimization of compound acidifiers using Box-Behnken design

试验旨在通过响应面法对复合酸化剂配方进行优化,筛选出最佳酸化剂配方。试验采用Response Surface中的Box-Behnken设计方法,以85%磷酸、一水柠檬酸、80%乳酸和磷酸氢二钠为复合酸化剂的主要原料组成,通过研究29个配方2%水溶液的缓冲力、系酸力改变量和10%水溶液的抑大肠杆菌菌圈直径,建立回归方程,并通过响应面分析法得到最优复合酸化剂配方。结果表明:1当以2%水溶液缓冲力为考察指标时,最佳配方为85%磷酸1.24%、一水柠檬酸40.00%、80%乳酸30.00%和磷酸氢二钠10.00%,此时2%水溶液缓冲能力最大,为4.78;2当以2%水溶液的系酸力改变量为考察指标时,最佳配方为85%磷酸5.00%、一水柠檬酸40.00%、80%乳酸30.00%和磷酸氢二钠1.02%,此时2%水溶液对饲料的系酸力改变量最大,为13.08;3当综合考虑2%水溶液缓冲能力和系酸力改变量时,最佳配方为85%磷酸5.00%、一水柠檬酸40.00%、80%乳酸30.00%和磷酸氢二钠2.28%。由此可见,考察指标不同导致复合酸化剂配方不同,通过响应面试验设计和分析方法可快速得到不同需求的复合酸化剂最优配方。

The Box- Behnken Design was used to study the formula of compound acidifiers. 29 different for-mulas, a compound with 85% phosphoric acid, citric acid monohydrate, 80% lactic acid and dibasic sodi-um phosphate for this experiment were studied based on the buffer capacity, acid-binding capacity changequantum of 2% solution and the bacteriostatic ability of 10% solution. A regression equation was set andthe optimum formula was determined with regression analysis method. Results indicated that the optimumeffects of buffer capacity occurred when the 85% phosphoric acid, citric acid monohydrate, 80% lactic acidand dibasic sodium phosphate of acidifier accounted for 1.24%, 40.00%, 30.00% and 10.00%, respective-ly, the optimum effects of acid-binding capacity change quantum occurred when the 85% phosphoric acid,citric acid monohydrate, 80% lactic acid and dibasic sodium phosphate of acidifier accounted for 5.00%,40.00%, 30.00% and 1.02%, respectively, while the optimum effects of both buffer capacity and acid-bind-ing capacity change quantum occurred when the 85% phosphoric acid, citric acid monohydrate, 80% lacticacid and dibasic sodium phosphate of acidifier accounted for 5.00%, 40.00%, 30.00% and 2.28%, respec-tively. It was concluded that the study of acidifier formula with the Box- Behnken design method was feasi-ble and the test efficiency of formula of acidifier study was improved with this method based on different ef-fect of acidifier.