慢性心理应激大鼠血浆及中枢β-EP、ACTH含量的变化及调肝治法方药的影响

The changes of β-endorphine and adrenocorticotrophin in brain and plasma of rats with chronic psychological stress and the effect of therapy of Gan (肝)-regulating prescription

目的 :观察慢性心理应激反应大鼠中枢及血浆 β 内啡肽 (β endorphine ,β EP)和促肾上腺皮质激素 (Adrenocorti cotrophin ,ACTH)含量的变化及调肝治法方药的影响。方法 :采用限制大鼠活动空间的方法 ,建立慢性心理应激反应模型。放射免疫法检测 β EP和ACTH。 结果 :模型大鼠下丘脑、垂体内 β EP水平明显降低 ,血浆含量明显升高 ;下丘脑、血浆ACTH水平均明显升高。调肝方药可以明显升高下丘脑、垂体内 β EP水平 ,降低血浆 β EP含量以及下丘脑和血浆ACTH水平。 结论 :调肝治法方药对慢性心理应激反应具有良好的调控作用 ,其机制与其调控中枢阿片促黑皮质激素原 (Proopiome lanocortin ,POMC)细胞分泌产物有关。

Objectives: To investigate the changes of β-endorphine (β-EP) and adrenocorticotrophin (ACTH) in plasma and brain of rats with chronic psychological stress and the effect of therapy of Gan (肝)-regulating prescription. Methods: To establish the chronic psychological stress model by bandaging rat and confining its activity space and detect the contents of β-EP and ACTH by RIA. Results: The lower content of β-EP had been observed in hypothalamus and pituitary of model rats, while higher in plasma. The contents of ACTH were increased in hypothalamus and plasma. The therapy of Gan(肝)-regnlating prescription could enhance the contents of β-EP in hypothalamus and pituitary, and decrease the contents of β-EP in plasma and ACTH in hypothalamus and plasma. Conclusions: The therapy of Gan(肝)-regnlating prescription could effectively regulate chronic psychological stress, the mechanism of which is involved with regulating the secretions of central proopiome-lanocortin.