14型肺炎球菌荚膜多糖-破伤风类毒素结合疫苗研究

Development of Pneumococcal capsular polysaccharide type 14-tetanus toxoid conjugate vaccines

目的 探讨制备肺炎球菌荚膜多糖 (PNCPS) 蛋白结合疫苗的适宜条件。方法 采用碳二亚胺法将 14型PNCPS与破伤风类毒素 (TT)结合 ,将结合物免疫NIH小鼠 ,用ELISA法检测小鼠血清中抗 14型PNCPS的IgG抗体滴度。结果 结合反应产率和结合物中多糖、蛋白含量的测定显示试验成功合成了 14型PNCPS TT结合物 ,该结合物具有 14型PNCPS的血清学特异性 ,将之免疫小鼠诱生的抗 14型PNCPS特异性IgG抗体水平显著高于单纯 14型PNCPS。结论 试验成功地合成了 14型PNCPS TT结合物 。

Objective To identify the reactive conditions for preparing PNCPS protein conjugates in order to cellect experiences in the development of conjugate vaccines afterwards. Methods 14 TT conjugates were prepared by carbodiimide mediated coupling of PNCPS with tetanus toxoid(TT). Female NIH mice were immunized with conjugates or pure PNCPS type 14, and the PNCPS antibodies in the sera of animals were detected by ELISA. Results The yield and composition of the conjugates tests showed that there are PNCPS and TT in conjugates. All of the conjugates elicited high level antibody response and induced immunogenic memory in mice, comparing to pure PNCPS. Conclusion 14 TT conjugates were successfully prepared with feasible technology.