分析5种结核杆菌耐药基因突变与耐药水平的关系

Identification of five drug resistance genes in Mycobacterium tuberculosis

目的 分析 5种结核杆菌 (M .tb)耐药基因突变的情况 ,了解基因突变和耐药水平的关系。方法 134例临床分离株均做传统梯度药敏试验和聚合酶链反应 单链构象多态性 (PCR SSCP)试验。结果 耐PZA(pncA)、SM(rpsL)、REP(rpoB)、INH (katG)、EMB(embB)基因突变率分别为 4 2 .7%、72 %、78%、6 9%和 4 3.9%。其中 ,上述高耐株基因突变率分别为 70 %、87.2 %、93.4 %、80 %、4 3.9%。低耐株分别为 12 .5 %、2 8.5 %、4 5 .4 %、18.7% ,EMB在低耐区无基因突变。结论 M .tb耐药基因突变与耐药水平联系密切。多数M .tb耐药基因突变易发生在高耐药区 。

Objective To study the relationship between drug resistance gene mutation and drug resistance level in M.tuberculosis. Methods 134 M.tuberculosis strains isolated from clinical sputum specimens were analyzed by PCR SSCP and classic drug susceptibility tests. Results The gene mutation rate of PZA, SM, RFP, INH and EMB resistance of isolated strains were 42.7%, 72%, 78%, 69% and 43.9% respectively, and the mutation rate of PZA, SM, RFP, INH and EMB higher concentration resistance isolated strains were 70%, 87.2% , 93.4%, 80% and 43.9% respectively, but 12.5%, 28.5%, 45.4% and 18.7% were the mutation rate of lower concentration resistance strains. Conclusion The gene mutation was related to drug resistance level of M.tuberculosis. The gene mutation rate was positively related to resistance.