p16和p53基因及顺铂联用对胆管癌细胞系QBC939的生长抑制作用

Inhibitory effects of gene p16 together with gene p53 and cisplatin to the growth of cholangiocarcinoma cell line QBC939

目的 :探索 p16基因和 p5 3基因及 p16基因和顺铂(CDDP)联合应用对体内外胆管癌细胞系生长的抑制作用 .方法 将重组体腺病毒 p16 (Ad- p16 )和 Ad- p5 3及 Ad- p16和CDDP联合作用于人胆管癌细胞系 QBC939,观察和分析其对体内外胆管癌细胞生长抑制作用及其机制 .结果 Ad- p16在 QBC939细胞中表达能抑制 QBC939细胞的生长 ,与 p5 3、CDDP联合应用能明显抑制 QBC939细胞的生长 ,p16和 p5 3基因诱导癌细胞发生 G1期阻滞和细胞凋亡 ,CDDP能诱导癌细胞发生 G2期阻滞和细胞凋亡 ;裸鼠皮下移植瘤模型瘤体内注射 Ad- p16及腹腔内注射 CDDP均能抑制 QBC939肿瘤的生长 ,两者联合应用具有协同作用 .结论 p16基因和 p5 3基因及 CDDP联合应用具有协同作用 .

AIM To explore inhibitory effects of recombinant adenoviruses p16 (Ad p16) with Ad p53 and Ad p16 with cisplatin (CDDP) to the growth of cholangiocarcinoma cell. METHODS The effects of Ad p16 with Ad p53 and Ad p16 with CDDP on the growth QBC939 cells in vitro and in vivo were analyzed. RESULTS Ad p16 expressed in cholangiocarcinoma cell line can inhibit growth of the QBC939 cells, while Ad p16 with Ad p53 and Ad p16 with CCDP together applied could significantly inhibit the growth of QBC939 cell in vitro and in vivo . The p16 with p53 transfected cells manifested apoptosis and G1 arrest, while CCDP induced G2 arrest and apoptosis. CONCLUSION p16 with p53 and p16 with CDDP can obviously increased the suppresson effeets on human cholangiocarcinoma cell line.