摘要
微筛法为多终点测试法,即除原噬菌体诱导外,增加了T_5抗性和氨苄青霉素抗性的前向突变,以及色氨酸回复突变。本文用微筛法测试了9类25种化合物,其中17种得到阳性结果,8种为阴性结果,与Ames试验的符合率达81％。本法在预测致癌原方面可能与Ames试验同样敏感。
Microscreen assay (MA) is a multi-endpoint assay which utilizes E. coli WP2s(λ). Endpoints thatcan be measured are: prophage induction (an indication of SOS induction), Trp^+ reversion(base pairsubstitution), and forward mutations to ampicillin resistance and resistance to phage T5. The genoto-xicity of 25 compounds which belong to 9 groups were measured with Microscreen assay. 17 compoundsgave positive results and 8 compounds gave negative results. The concordance between Microscreen assayand Ames test was 81％. It appears that Microscreen assay is a litter more senstive than Ames test inprediction of carcinogens.
出处
《北京医科大学学报》
CSCD
1992年第1期42-44,共3页
Journal of Peking University(Health Sciences)
关键词
微筛法
原噬菌体诱导
遗传毒性
Microscreen assay
Prophage induction
T5 resistance
Ampicillin resistance
Trp^+reveTS 1on
