黄曲霉毒素B_1诱发大鼠肝癌的实验病理研究

THE STUDY OF EXPERIMENTAL PATHOLOGY OF AFLATOXIN B_1-INITIATED HEPATOCARCINOGENISIS IN RATS

本实验以黄曲霉毒素B_1(AFB_1)诱癌,苯巴比妥钠(PB)和四氯化碳(CCl_4)促癌,建立了一种新的诱发大鼠肝癌的实验模型,并用病理组织学、形态定量测算及电子显微镜等方法,研究了癌变过程中变异肝细胞灶/给节的发生和发展及其与肝癌的关系。结果表明,PB和CCl_4能使变异细胞灶的数目和体积增加,而且可促进肝癌的发生。作者还发现,嗜酸细胞结节与高分化型肝细胞癌密切相关。

The hepatocarcinomas were induced by aflatoxin B, (AFB_1) and Fromoted by phenobarbital(PB)and carbon tetrachloride (Cl_4). Using histopathology, measure of morphormetry and electromicro-scopy, we studied the occurence and progression of altered hepatocyte foci/nodules, and the correla-tions between altered nodules and liver cancer. The results showed that PB and CCl_4 were able toenhance the average number and volume of altered foci/nodules, and to make the liver cancer occurearlier. Otherwise, it was obviouse that eosiphilic nodules may develop into well differetiated livercancer. A new experimental model of AFB_1 induced rat liver cancer was established in our protocol.