气相色谱法测定三类氢化油脂反式脂肪酸的含量

Determination of the trans-fatty acids in three kinds of hydrogenated fat by gas chromatography

通过气相色谱法对市售三类氢化油脂的反式脂肪酸含量进行了测定,方法的重复性好,最低检出限能够满足要求。结果表明,三类氢化油脂反式脂肪酸的平均含量方别为:人造奶油(0.70g/100g),植物奶油(0.36g/100g)和(1.12g/100g)。所测定的样品中,以C_(18)为主的反式脂肪酸中一烯酸和二烯酸含量较高,而三烯酸的反式甚微。

Trans-fatty acids(TFA) in three kinds of commercially available hydrogenated fat was determined by gas chromatography. The method had high precision with requisite minimum detection limit. The results showed that the average TFA content of them were as follows: margarine(0.70g/100g), vegetable cream(0.36g/100g), and shortening(6.2%). The major TFA in the samples were trans C_(18), among which, trans C_(18:1) and C_(18:2) exhibited the more content, followed by small amounts of trans C_(18:3).