高效液相色谱法测定纳米海绵中游离甲醛含量

Determination of Free Formaldehyde in Nano-sponge by High Performance Liquid Chromatography

建立了高效液相色谱法(high performance liquid chromatography,HPLC)测定纳米海绵中游离甲醛含量的分析方法。以2, 4-二硝基苯肼为衍生剂与甲醛反应生成2, 4-二硝基苯腙。在352nm波长处,HPLC测定,外标法定量。该方法在0.02～20mg/L浓度范围内具有良好线性,相关系数(r^2)为0.9999,相对标准偏差小于5%。该方法操作简单、灵敏度高、重复性好,可用于纳米海绵中游离甲醛含量的测定。

To establish a method for determination of free formaldehyde content in nano-sponge by high performance liquid chromatography(HPLC).The 2,4-dinitrophenylhydrazine was used as a derivatizing agent to react with formaldehyde to form 2,4-dinitrophenylhydrazine.At the wavelength of 352 nm,the sample was determined by HPLC and quantified by external standard method.This method had good linearity in the concentration range of 0.02-20 mg/L,the correlation coefficient(r^2)was 0.9999,and the relative standard deviation(RSD)was less than 5%.This method is simple in operation,high in sensitivity and good in repeatability,which can be used for the determination of free formaldehyde content in nano-sponges.